Pan T Cell Isolation Kit,mouse

T and B cell Isolation

with 5 ml of PBS and add to gradient.  Centrifuge at 300 g for 40 minutes, room temperature.  Collect the B cells at the interface. Wash 3 times with PBS.  Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes.  Aspirate all of the supe

Isolation of Mouse Thymic Dendritic Cell Precursors

Dendritic cells (DC) are efficient antigen-presenting cells. Their ability to present antigens via MHC class I and MHC class II molecules to T cells allows them not only to initiate an immune response to exogenous pathogens but also to induce

Mouse B cell isolation with magnetic beads

实验概要 Isolate B cells from total mouse cells   Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room