- 询价
- elatins
- JLC260894
- 上海
- 2026年03月10日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-ITGA2 antibody
- 宿主：
Rabbit
- 浓度：
0.4 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC260894
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ITGA2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human ITGA2
Reactivity Human
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ITGA2
Full name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Synonyms BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
Swissprot P17301
Target Background
This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human gasrtic cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using ml260894(ITGA2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml260894(ITGA2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:129 kDa
Positive control:A431 cells
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: A431 cells
Primary antibody: ml260894(ITGA2 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 30 seconds
ELISA
Recommended dilution: 1000-2000
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文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
