Product overview Description Anti-AKR1B1 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Synthetic peptide of human AKR1B1 Reactivity Human Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol AKR1B1 Full name Aldo-keto reductase family 1, member B1 (aldose reductase) Synonyms AR; ADR; ALR2; ALDR1 Swissprot P15121
Target Background This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human colon cancer Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human ovarian cancer Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml260849(AKR1B1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml260849(AKR1B1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Western blotting Predicted band size:36 kDa Positive control:Hela cells Recommended dilution: 500-2000
Gel: 10%SDS-PAGE Lysate: 27 μg Lane: Hela cells Primary antibody: ml260849(AKR1B1 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second