- 询价
- gelatins
- JLC260626
- 上海
- 2025年07月11日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-MMP25 antibody
- 宿主：
Rabbit
- 浓度：
0.6 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-MMP25 antibody
Cat. No. JLC260626
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-MMP25 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human MMP25
Reactivity Human
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MMP25
Full name matrix metallopeptidase 25
Synonyms MMP20, MMPL1, MMP-25, MMP20A, MT6MMP, MTMMP6, MT-MMP6, MT6-MMP, MT-MMP 6
Swissprot Q9NPA2
Target Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml260626(MMP25 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml260626(MMP25 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:63 kDa
Positive control:823 cells
Recommended dilution: 500-2000
Gel: 10+12%SDS-PAGE
Lysate: 40 μg
Lane: 823 cells
Primary antibody: ml260626(MMP25 Antibody) at dilution 1/1300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 2000-5000
Cat. No. JLC260626
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-MMP25 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human MMP25
Reactivity Human
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MMP25
Full name matrix metallopeptidase 25
Synonyms MMP20, MMPL1, MMP-25, MMP20A, MT6MMP, MTMMP6, MT-MMP6, MT6-MMP, MT-MMP 6
Swissprot Q9NPA2
Target Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml260626(MMP25 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml260626(MMP25 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:63 kDa
Positive control:823 cells
Recommended dilution: 500-2000
Gel: 10+12%SDS-PAGE
Lysate: 40 μg
Lane: 823 cells
Primary antibody: ml260626(MMP25 Antibody) at dilution 1/1300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 2000-5000
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Anti-MMP25 antibody
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