Product overview Description Anti-RAN rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Synthetic peptide of human RAN Reactivity Human, Mouse, Rat Content 0.6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol RAN Full name Ras-related nuclear protein Synonyms TC4, Gsp1, ARA24 Swissprot P62826
Target Background RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Applications Immunohistochemistry Predicted cell location: Cytoplasm, Nucleus Positive control: Human lung cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml260082(RAN Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Western blotting Predicted band size:25 kDa Positive control:Hela, NIH/3T3 and HepG2 cells, Mouse testis tissue Recommended dilution: 200-1000
Gel: 10%SDS-PAGE Lysate: 30 μg Lane 1-4: Hela cells, NIH/3T3 cells, HepG2 cells, Mouse testis tissue Primary antibody: ml260082(RAN Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute