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文献和实验A novel method of growing fungi for DNA extraction
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tubes (Cenis 1992 Nucl. Acids Res. 20:2380). Growing fungal cultures in liquid may require
to facilitate genomic DNA purification. See figure below for details. Step 1–DNA Capture–Cells are lysed and crude DNA is captured on magnetic beads leaving most of the cell debris and protein behind in solution. Step 2–DNA Purification–
. The tubes are then incubated at a temperature of 37 - 55ºC (optimal temp for Proteinase K activity) overnight in a water bath or incubator. During this step the white blood cells' membranes are denatured and DNA goes out in solution.
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