- 询价
- gelatins
- JLC225470
- 上海
- 2025年07月09日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-ECD antibody
- 宿主：
Rabbit
- 浓度：
0.96 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-ECD antibody
Cat. No. JLC225470
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ECD rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ECD
Reactivity Human
Content 0.96 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ECD
Full name ecdysoneless cell cycle regulator
Synonyms GCR2; SGT1; HSGT1
Swissprot O95905
Target Background
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human tonsil
Recommended dilution: 40-200 Predicted cell location: Cytoplasm or Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 40-200
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml225470(ECD Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225470(ECD Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:73 kDa
Positive control:Hela cell lysate
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Hela cell lysate
Primary antibody: ml225470(ECD Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 5000-10000
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Anti-ECD antibody
询价
