ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
42kDa
细胞定位
细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Paralemmin 1
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: Paralemmin, also called Paralemmin-1 or PALM, is a widely expressed peripheral membrane protein that is involved in cell structure and shape. A hydrophobic protein, Paralemmin is anchored to the cytoplasmic side of the cell membrane via di-palmitoylation and prenylation of its C-terminal cysteine cluster. Functioning at the synapse to regulate neuronal plasticity and plasma membrane dynamics, Paralemmin can bind to the dopamine receptor D3, thereby reducing D3 expression and subsequent adenylate cyclase activity. Overexpression of Paralemmin induces fibroblasts to extend long filopodia and to assume extreme cell shapes, suggesting involvement in the formation and stabilization of the plasma membrane. Two isoforms of Paralemmin exists due to alternative splicing events.
Function: Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner.