Product overview
Description Anti-MAPKAPK2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human MAPKAPK2
Reactivity Human, Mouse
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MAPKAPK2
Full name mitogen-activated protein kinase-activated protein kinase 2
Synonyms MK2; MK-2; MAPKAP-K2
Swissprot P49137
Target Background
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml261888(MAPKAPK2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml261888(MAPKAPK2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:46 kDa
Positive control:A549 cells
Recommended dilution: 200-1000