Product overview Description Anti-NARF rabbit polyclonal antibody Applications ELISA, IHC Immunogen Fusion protein of human NARF Reactivity Human, Mouse, Rat Content 1.56 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol NARF Full name nuclear prelamin A recognition factor Synonyms IOP2 Swissprot Q9UHQ1
Target Background Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Applications Immunohistochemistry Predicted cell location: Nucleus Positive control: Human ovarian cancer Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml224946(NARF Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200)