Product overview
Description Anti-CSTB rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human CSTB
Reactivity Human, Mouse
Content 0.7 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CSTB
Full name cystatin B (stefin B)
Synonyms PME; ULD; CST6; EPM1; STFB; EPM1A
Swissprot P04080
Target Background
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human brain
Recommended dilution: 25-100 Predicted cell location: Cytoplasm and Nucleus
Positive control: Human gastric cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml261602(CSTB Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml261602(CSTB Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:11 kDa
Positive control:Mouse spleen tissue
Recommended dilution: 200-1000