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- 详细信息
- 文献和实验
- 技术资料
- 库存:
500-P303BT
- 靶点:
生物素标记TRAIL
- 级别:
科研
- 目录编号:
500-P303BT
- 克隆性:
多克隆
- 抗原来源:
20.0 kDa Recombinant Murine TRAIL (PeproTech catalog# 315-19)
- 抗体英文名:
Biotinylated Anti-Murine TRAIL
- 抗体名:
生物素标记TRAIL
- 标记物:
生物素标记TRAIL
- 宿主:
兔
- 适应物种:
小鼠
- 应用范围:
WB ELISA
- 浓度:
Biotinylated Anti-Murine TRAIL
- 规格:
25ug
产品编号: 500-P303BT
资料来源:多克隆兔
制备:从用高纯度重组鼠TRAIL免疫的兔子血清中制备。通过亲和层析纯化抗鼠TRAIL特异性抗体,然后进行生物素化。
免疫原:大肠杆菌衍生的20.0 kDa重组小鼠TRAIL(PeproTech目录#315-19)
夹心ELISA:为了通过夹心ELISA(使用100毫升/孔)检测小鼠TRAIL,需要浓度为0.25–1.0微克/毫升的这种抗体。该生物素化多克隆抗体与PeproTech的多克隆抗鼠TRAIL(500-P303)结合作为捕获抗体,允许检测至少0.2–0.4 ng/孔的重组鼠TRAIL。
生物素化抗鼠TRAIL夹心ELISA
Western Blot:为了通过Western Blot分析检测小鼠TRAIL,该抗体可在0.1-0.2 mg/ml的浓度下使用。当与兼容的开发试剂一起使用时,在还原或非还原条件下,重组小鼠TRAIL的检测限为1.5-3.0 ng/lane。
| 500-P303-50 | Rabbit Anti-Murine TRAIL | 50ug |
| 500-P303-100 | Rabbit Anti-Murine TRAIL | 100ug |
| 500-P303-1000 | Rabbit Anti-Murine TRAIL | 1000ug |
| 500-P303Bt-25 | Biotinylated Rabbit Anti-Murine TRAIL | 25ug |
| 500-P303Bt-50 | Biotinylated Rabbit Anti-Murine TRAIL | 50ug |
| 500-P303Bt-1000 | Biotinylated Rabbit Anti-Murine TRAIL | 1000ug |
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文献和实验Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy
activity of TRAIL such that larger, more established tumors could be eradicated as efficiently as smaller tumors. The information presented here describes the production of an adenoviral vector engineered to carry the complementary DNA (cDNA) for murine
Biochemical Analysis of the Native TRAIL Death-Inducing Signaling Complex
receptor 1 (TRAIL-R1/DR4), TRAIL-R2 (KILLER/APO-2/DR5/TRICK), CD95 (Fas, APO-1), or TNF receptor 1 (TNF-R1), this complex is termed death-inducing signaling complex (DISC). The compositions of the various DISCs have been intensively studied in the last 12
大鼠肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4 )ELISA试剂盒 说明书
生物素化的抗大鼠 TRAIL R4 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值, TRAIL R4 浓度与 OD 值成正比,可通过绘制标准曲线求出标本中 TRAIL R4 浓度。 试剂盒组成 ( 2-8℃保存) 酶标 板( Coated Wells ) 96 孔
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