Product overview
Description Anti-PCK1 rabbit polyclonal antibody
Applications ELISA, WB
Immunogen Synthetic peptide of human PCK1
Reactivity Human, Mouse, Rat
Content 1 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PCK1
Full name phosphoenolpyruvate carboxykinase 1 (soluble)
Synonyms PEPCK1; PEPCKC; PEPCK-C
Swissprot P35558
Target Background
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
Applications
Western blotting
Predicted band size:69 kDa
Positive control:Mouse liver and kidney tissue
Recommended dilution: 500-2000