Product overview Description Anti-CA7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Full length fusion protein Reactivity Human, Mouse Content 0.8 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol CA7 Full name carbonic anhydrase 7 Synonyms CAVII Swissprot P43166
Target Background Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms.
Applications Immunohistochemistry Predicted cell location: Cytoplasm or Nucleus Positive control: Human gastric cancer Recommended dilution: 50-300 Predicted cell location: Cytoplasm or Nucleus Positive control: Human thyroid cancer Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml224370(CA7 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml224370(CA7 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:30 kDa Positive control:Human urinary bladder tissue,Mouse heart tissue,Mouse kidney tissue and RAW264.7 cell lysates Recommended dilution: 500-2000
Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-4: Human urinary bladder tissue,Mouse heart tissue,Mouse kidney tissue and RAW264.7 cell lysates Primary antibody: ml224370(CA7 Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds