Product overview Description Anti-COA7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Full length fusion protein Reactivity Human, Mouse Content 0.9 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol COA7 Full name cytochrome c oxidase assembly factor 7 (putative) Synonyms RESA1; SELRC1; C1orf163 Swissprot Q96BR5
Target Background The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. COA7 (cytochrome c oxidase assembly factor 7), also known as RESA1, SELRC1 or C1orf163, is a 231 amino acid mitochondrial protein that belongs to the hcp beta-lactamase family. Consisting of five Sel1-like repeats, COA7 may be associated with respiratory chain assembly. COA7 is encoded by a gene located on human chromosome 1p32.3. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene, which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration.
Applications Immunohistochemistry Predicted cell location: Cytoplasm or Cell membrane Positive control: Human thyroid cancer Recommended dilution: 40-200 Predicted cell location: Cytoplasm or Cell membrane Positive control: Human esophagus cancer Recommended dilution: 40-200
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml224337(COA7 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml224337(COA7 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:26 kDa Positive control:HL-60 and HEPG2 cell lysates Recommended dilution: 500-2000
Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: HL-60 and HEPG2 cell lysates Primary antibody: ml224337(COA7 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds