Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-98
94
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
92-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.