Product overview Description Anti-ZKSCAN1 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human ZKSCAN1 Reactivity Human, Mouse, Rat Content 0.7 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol ZKSCAN1 Full name zinc finger with KRAB and SCAN domains 1 Synonyms KOX18; ZNF36; PHZ-37; ZNF139; ZSCAN33; 9130423L19Rik Swissprot P17029
Target Background The ZKSCAN1 gene encodes a transcriptional regulator of the KRAB (Kruppel-associated box) subfamily of zinc finger proteins, which contain repeated Cys2-His2 (C2H2) zinc finger domains that are connected by conserved sequences, called H/C links (summarized by Tommerup and Vissing, 1995 [PubMed 7557990]). Transcriptional regulatory proteins containing tandemly repeated zinc finger domains are thought to be involved in both normal and abnormal cellular proliferation and differentiation. See ZNF91 (MIM 603971) for general information on zinc finger proteins.
Applications Immunohistochemistry Predicted cell location: Nucleus and Cytoplasm Positive control: Human tonsil Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml223989(ZKSCAN1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:64 kDa Positive control:Human cerebrum tissue lysate Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Human cerebrum tissue lysate Primary antibody: ml223989(ZKSCAN1 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds