Product overview Description Anti-GSTM4 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Full length fusion protein Reactivity Human, Mouse Content 0.6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol GSTM4 Full name glutathione S-transferase mu 4 Synonyms GTM4; GSTM4-4 Swissprot Q03013
Target Background Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds. Multiple transcript variants, each encoding a distinct protein isoform, have been identified.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human thyroid cancer Recommended dilution: 10-50 Predicted cell location: Cytoplasm Positive control: Human lung cancer Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml223890(GSTM4 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml223890(GSTM4 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:26 kDa Positive control:Mouse liver tissue,Mouse lung tissue Recommended dilution: 200-1000
Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Mouse liver tissue,Mouse lung tissue Primary antibody: ml223890(GSTM4 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds