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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Thioredoxin domain-containing protein 12 (Txndc12)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4164.0 |
| 规格: | 20μg | 产品价格: | ¥2208.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Txndc12Uniprot No.:
Q9CQU0别名:
Endoplasmic reticulum resident protein 19 Short name: ER protein 19 Short name: ERp19 Thioredoxin-like protein p19种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
18.5 kDa表达区域:
25-170aa氨基酸序列:
RTGLGKGFGDHIHWRTLEDGKKEAAASGLPLMVIIHKSWCGACKALKPKFAESTEISELSHNFVMVNLEDEEEPRDEDFSPDGGYIPRILFLDPSGKVRPEIINESGNPSYKYFYVSAEQVVQGMKEAQERLTGDAFREKHFQDEL蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Possesses significant protein thiol-disulfide oxidase activity.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"ERp19 and ERp46, new members of the thioredoxin family of endoplasmic reticulum proteins."Knoblach B., Keller B.O., Groenendyk J., Aldred S., Zheng J., Lemire B.D., Li L., Michalak M.Mol. Cell. Proteomics 2:1104-1119(2003)Function:
Possesses significant protein thiol-disulfide oxidase activity.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags
quantification of Trx target disulfides in complex protein extracts is described. The procedure utilizes the isotope-coded affinity tag (ICAT) reagents containing a thiol reactive iodoacetamide group and a biotin affinity tag to target peptides containing reduced
Purification of KHC Motor Domain Protein
Purification of KHC Motor Domain Protein Induced cells (2 - 5 g pellet of pET/KHC in BL21(DE3)pLysS host cells) HEM buffer = 10 mM HEPES pH 7.2 1 mM DTT 1 mM MgCL2 1 mM
Purification of Ncd Motor Domain Protein
Purification of Ncd Motor Domain Protein Induced cells (2 - 5 g pellet of pET/Ncd in BL21(DE3)pLysS host cells) HEM buffer = 10 mM HEPES pH 7.2 1 mM DTT 1 mM MgCL2 1 mM
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