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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant mouse platelet factor 4 (Pf4)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant mouse platelet factor 4 (Pf4)品名简称:
Recombinant Mouse Pf4 protein货号:
CSB-EP017809MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Pf4Uniprot No.:
Q9Z126别名:
C-X-C motif chemokine 4种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
24.2 kDa表达区域:
30-105aa氨基酸序列:
VTSAGPEESDGDLSCVCVKTISSGIHLKHITSLEVIKAGRHCAVPQLIATLKNGRKICLDRQAPLYKKVIKKILES蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).功能2:
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).文献:
"Significantly elevated expression of PF4 (platelet factor 4) and eotaxin in the NOA mouse, a model for atopic dermatitis."Watanabe O., Natori K., Tamari M., Shiomoto Y., Kubo S., Nakamura Y.J. Hum. Genet. 44:173-176(1999)研究领域:
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文献和实验Using the Gibbs Motif Sampler to Find Conserved Domains in DNA and Protein Sequences
program. Keywords: Gibbs sampling; Transcription factor binding site; Sequence Alignment; Motif; DNA; Protein; Phylogentic Footprinting; Stochastic Algorithm; Markov Chain Monte?Carlo; Bayesian statistics
Searching for Non‐B DNA‐Forming Motifs Using nBMST (Non‐B DNA Motif Search Tool)
Foiry, L., Dong, L., Savouret, C., Hubert, L., te Riele, H., Junien, C., and Gourdon, G. 2006. Msh3 is a limiting factor in the formation of intergenerational CTG expansions in DM1 transgenic mice. Hum. Genet. 119:520‐526
Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
量的 ChIP 数据却非常具有挑战性。在每个关键步骤都有很多需要注意的细节。下面是 Active Motif ChIP 实验专家根据多年经验总结的每个步骤中的原理及一些需要注意的细节。第一步 染色质交联为什么要用甲醛交联?ChIP 技术主要是一种解码信息(目的蛋白在染色质上的分布)的方法。对于解码信息,首先就需要确保信息保存下来而不失真,ChIP 技术用到的最主要的信息保存方式是甲醛交联。甲醛交联也是 ChIP 实验非常关键的一个步骤。最早的 ChIP 技术是不用甲醛交联的[6],只是后来发现甲醛交联能更好的保存蛋白
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