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ompT/ompT蛋白/Omptin Outer membr

ane protein 3B Protease A Protease VII蛋白/Recombinant Escherichia coli O157:H7 Protease 7 (ompT) (G236K,K237G)重组蛋白
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  • ¥69 - 699
  • CUSABIO已认证
  • CN
  • CSB-EP348292EOD(M)
  • 2025年07月05日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 保质期

      12个月

    • 英文名

      Recombinant Escherichia coli O157:H7 Protease 7 (ompT) (G236K,K237G)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      10ug/20ug/50ug/100ug/200ug/500ug/1mg

    规格:10ug产品价格:¥69.0
    规格:20ug产品价格:¥139.0
    规格:50ug产品价格:¥209.0
    规格:100ug产品价格:¥349.0
    规格:200ug产品价格:¥419.0
    规格:500ug产品价格:¥559.0
    规格:1mg产品价格:¥699.0

    Research Areas

    Microbiology

    Uniprot ID

    P58603

    Gene Names

    ompT

    Alternative Name(s)

    Omptin Outer membrane protein 3B Protease A Protease VII

    Lead Time

    3-7 business days

    Organism

    Escherichia coli O157:H7

    Source

    E.coli

    Expression Region

    21-317aa(G236K,K237G)

    Protein Length

    Full Length of Mature Protein

    Tag Info

    N-terminal 6xHis-SUMO-tagged

    Target Protein Sequence

    STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVEASDNDEHYDPKGRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRVTNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF

    MW

    49.5 kDa

    Purity

    Greater than 90% as determined by SDS-PAGE.

    Form

    Liquid or Lyophilized powder

    Buffer

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Notes

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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    • Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases

      that can be used to determine the topology of an envelope membrane protein is to apply the “dual protease” strategy. This approach involves several steps: first, performing an in vitro import assay; second, applying a “dual protease” protection assay using

    • 蛋白质组学指南

      -femtomolar level if enough radioactivity can be incorporated into the phosphoprotein of interest. In our hands, this can be as little as 1,000 cpm (not ideal). Briefly, a 32 P-labeled protein is digested with a protease and the resulting phosphopeptides

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    文献支持
    ompT/ompT蛋白/Omptin Outer membrane protein 3B Protease A Protease VII蛋白/Recombinant Escherichia coli O157:H7 Protease 7 (ompT) (G236K,K237G)重组蛋白
    ¥69 - 699