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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dog MMP13 protein (MMP13)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dog MMP13 protein (MMP13)品名简称:
Recombinant Dog MMP13 protein货号:
CSB-EP2042DO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
MMP13Uniprot No.:
A0A0C6E3R7别名:
/种属:
Canis lupus familiaris (Dog) (Canis familiaris)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
67.5 kDa表达区域:
28-478aa氨基酸序列:
LPLPSDGDDDLSEEDFQLAERYLKSYYYPLNPAGILKKSAAGSVADRLREMQSFFGLEVTGKLDDNTLDIMKKPRCGVPDVGEYNVFPRTLKWSKTNLTYRIVNYTPDLTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPRHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSRDLIFIFRGRKYWALNGYDILEGYPQKISELGFPKEVKKISAAVHFEDTGKTLFFSGNQVWSYDDTNQIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYNIWSKRIVRVMPANSLLWC蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Expression of MMP13 in canine mammary tumor."Kumar B.V.S.Submitted (FEB-2015)研究领域:
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文献和实验IL-1β诱导软骨细胞炎症模型:浓度、时间点和检测指标怎么选?
-1β + 药物低剂量 ·IL-1β + 药物中剂量 ·IL-1β + 药物高剂量 推荐检测: ·CCK-8 ·IL-6、COX-2、iNOS ·COL2、Aggrecan ·MMP13、ADAMTS5 ·IF 或 WB 验证关键蛋白 3. 做外泌体或材料浸提液 建议先做: ·细胞毒性; ·剂量梯度; ·摄取实验或作用时间摸索; ·再进入 IL-1β 模型。 推荐检测: ·CCK-8 ·炎症因子 ·基质代谢指标 ·免疫荧光 ·qPCR/WB 4. 和动物模型结果对应 动物端常见指标: ·番红固绿 ·
骨关节炎(OA)动物实验后,组织学评价怎么做?4 类染色一次讲清
减弱; ·OARSI 评分是否升高。 2. 判断药物是否保护软骨 推荐组合: ·HE; ·番红固绿; ·COL2; ·Aggrecan; ·MMP13; ·ADAMTS5。 重点观察: ·软骨结构是否改善; ·蛋白多糖是否保留; ·COL2/Aggrecan 是否回升; ·MMP13/ADAMTS5 是否下降。 3. 判断抗炎作用 推荐组合: ·HE; ·滑膜 HE; ·IL-1β; ·TNF-α; ·IL-6; ·COX-2; ·iNOS。 重点观察: ·滑膜是否增生; ·炎症
骨关节炎(OA)动物模型选择指南:DMM、ACLT、MIA 怎么用?
怎么选? 1. 研究慢性 OA 发生机制 优先选择:DMM 适合搭配: ·小鼠模型; ·4–8 周或更长观察周期; ·番红固绿 + OARSI 评分; ·COL2、Aggrecan、MMP13、ADAMTS5; ·qPCR/WB/IHC/IF; ·必要时加 micro-CT。 适合课题: ·软骨细胞衰老; ·炎症通路; ·软骨基质代谢; ·线粒体功能; ·焦亡、铁死亡、自噬; ·基因敲除或病毒干预。 2. 研究创伤后 OA 或关节不稳 优先选择:ACLT 适合搭配: ·大鼠或兔模型; ·
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