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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Transposase insE for insertion sequence IS3A (insE1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥35280.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥8400.0 |
| 规格: | 20μg | 产品价格: | ¥3000.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
insE1Uniprot No.:
P0CF66别名:
insE1; b0298; JW5036; Transposase InsE for insertion sequence IS3A种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
Mammalian cell分子量:
37.5 kDa表达区域:
1-99aa氨基酸序列:
MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK蛋白标签:
C-terminal hFc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Involved in the transposition of the insertion sequence IS3.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
MicrobiologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"The complete genome sequence of Escherichia coli K-12."Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)Function:
Involved in the transposition of the insertion sequence IS3.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons
for procedures such as insertional mutagenesis, shotgun sequencing, or the generation of protein and transcriptional fusions for subsequent analysis. Active transposase can readily be generated following expression in Escherichia coli , and efficiencies of 10−
://bioinfo.md.huji.ac.il/marg/promec Escherichia coli mRNA promoters with experimentally-identified transcriptional start sites 56. RRNDB http://rrndb.cme.msu.edu/ Variation in prokaryotic ribosomal RNA operons 57. rSNP Guide http://util.bionet.nsc.ru
and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers
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