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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Bifunctional protein HldE (hldE)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Bifunctional protein HldE (hldE)品名简称:
Recombinant E.coli hldE protein货号:
CSB-EP302846ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
hldEUniprot No.:
P76658别名:
Including the following 2 domains: D-beta-D-heptose 7-phosphate kinase (EC:2.7.1.167) Alternative name(s): D-beta-D-heptose 7-phosphotransferase D-glycero-beta-D-manno-heptose-7-phosphate kinase D-beta-D-heptose 1-phosphate adenylyltransferase (EC:2.7.7.70) Alternative name(s): D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
E.coli分子量:
67.1 kDa表达区域:
1-477aa氨基酸序列:
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.功能2:
Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.文献:
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)研究领域:
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文献和实验-3b, sharing97% sequence similarity within their kinase catalytic domains(2) . In contrast to most kinases, GSK-3 is inactivated by phosphorylation(2) . GSK-3 is active in nonstimulated cells, whereasother kinases require cell stimulation for activation
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, which is very convenient when black-and-white photography is used for documentation. Use the Sigma FAST DAB with metal enhancer (Sigma D0426), following the supplier’s instructions, but shorten the metal amplification step to ~1 min. Problem: In Giemsa-stained
Crystallization of Kinesin Family Motor Proteins
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human,rat and Neurospora (Kull et al.,1996; Sack et al.,1997; Song et al.,2001)and dimeric rat Kinesin-1 (two motor domains connected
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