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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human DDB1- and CUL4-associated factor 16 (DCAF16)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human DDB1- and CUL4-associated factor 16 (DCAF16)品名简称:
Recombinant Human DCAF16 protein货号:
CSB-EP865168HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
DCAF16Uniprot No.:
Q9NXF7别名:
C4orf30种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
31.2 kDa表达区域:
1-216aa氨基酸序列:
MGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVPNLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSHCVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRATPIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.功能2:
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.文献:
"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006)研究领域:
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文献和实验Immunity:厦门大学韩家淮团队揭示 PELO 控制 NOD 样受体家族蛋白寡聚化组装和激活的机制
的 ATPase 活性,控制其寡聚化组装和激活。 PELO 是进化上高度保守的蛋白,参与核糖体相关质量控制(ribosome-associated quality control,RQC),其与 HBS1L 形成的复合物可以识别具有空解码中心(decoding center)的停滞核糖体(stalled ribosome),进而介导停滞核糖体的 40S 亚基和 60S 亚基分离,促进核糖体的回收利用(ribosome rescue)【4,5】。因而,PELO 在胚胎发育、精子生成、表皮内稳态及神经
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
[4] 。由于在信号转导和表观遗传研究中的突出作用,ChIP 在肿瘤 [5-7] 、神经科学 [8-10] 、植物发育 [11-13] 等领域中应用非常广泛,同时有关细胞凋亡 [14] 、雌激素信号转导 [15] 、胰岛素抵抗 [16] 、组织发育 [1] 的文献中也用到ChIP。 目前,最常见的有以下两种 ChIP 实验技术: nChIP:用来研究 DNA 及高结合力蛋白,采用微球菌核酸酶(micrococcal nuclease)消化染色质,然后进行片段富集及后续分析,适用于组蛋白及其异构体,例如 [17
相关分子模式(pathogen-associated molecular patterns,PAMPs)和损伤相关的分子模式 (damage associated molecular patterns, DAMPs)。通过识别侵入病原的 PAMPs(如 LPS,脂磷壁酸,鞭毛蛋白等)和内源的损伤或死亡细胞的 DAMPs(如 ATP,尿酸结晶,S100 等),胞膜上的 Toll 样受体(TLR)和胞内的 Nod 样受体(NLR)被激活。细菌的感染通常伴有组织的损伤释放的宿主内源成分。因而 PAMPs
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