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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla)品名简称:
Recombinant Pseudomonas aeruginosa bla protein货号:
CSB-EP321251EZW规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
blaUniprot No.:
P14489别名:
Beta-lactamase PSE-2种属:
Pseudomonas aeruginosa蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
43.5 kDa表达区域:
21-266aa氨基酸序列:
SITENTSWNKEFSAEAVNGVFVLCKSSSKSCATNDLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQWERDLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISAVNQVEFLESLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWWVGWVEKETEVYFFAFNMDIDNESKLPLRKSIPTKIMESEGIIGG蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hydrolyzes both carbenicillin and oxacillin.功能2:
Hydrolyzes both carbenicillin and oxacillin.文献:
"Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.Structure 8:1289-1298(2000)研究领域:
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文献和实验生长和活力的目标蛋白的 pET 重组体。带有 pLacI 的宿主菌产生额外的抑制 pETBlue 和 pTriEx 载体基础表达的 lac 阻遏蛋白。 λ DE3 溶原化试剂盒用于制备其它遗传背景的新表达宿主菌。 AD494 菌株为硫氧还蛋白还原酶 ( trxB ) 突变菌株,能够在胞浆内形成二硫键,提供了生产正确折迭的活性蛋白的潜力。 TrxB 突变可用卡那霉素选择,因此该菌株建议用于带氨苄抗性标记 bla 的质粒。 B834 为 BL21 的亲本菌株。这些蛋白
铜绿假单胞菌(Pseudomonas aeruginosa,PA)又称绿脓杆菌,是引起急性或慢性感染的最常见的条件致病菌之一,由其引起的院内感染往往治疗难度极大,几乎具有目前已知的细菌主要耐药机制,已成为引起院内获得性肺炎多重耐药革兰阴性菌的代表。PA 感染是治疗的难题,归其原因,在于其广泛而多重的耐药性。因此,了解铜绿假单胞菌的耐药相关基因的情况,有助于临床研发更有效的治疗铜绿假单胞菌感染性疾病的抗菌药物。PA 的耐药机制概括起来主要有以下几个方面:各种外排泵系统的过度
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