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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Serotonin N-acetyltransferase (AANAT)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Serotonin N-acetyltransferase (AANAT)品名简称:
Recombinant Human AANAT protein货号:
CSB-EP623090HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
AANATUniprot No.:
Q16613别名:
Aralkylamine N-acetyltransferase种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
51.8 kDa表达区域:
1-207aa氨基酸序列:
MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC蛋白标签:
N-terminal 10xHis-GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.功能2:
Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.文献:
"cAMP regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation." Coon S.L., Weller J.L., Korf H.-W., Namboodiri M.A., Rollag M., Klein D.C. J. Biol. Chem. 276:24097-24107(2001)研究领域:
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文献和实验Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is a common protein �modification process in eukaryotic cells. Despite its widespread occurrence, the biological significance of this modification process
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
. 4‐(2'‐Methoxyphenyl)‐1‐[2'‐[N‐(2″‐pyridinyl)‐p‐iodobenzamido]ethyl] piperazine and 4‐(2'‐methoxyphenyl)‐1‐[2'‐[N‐(2″‐pyridinyl)‐p‐fluorobenzamido]ethyl]piperazine, two new antagonists at pre‐and postsynaptic serotonin‐1A receptors. J. Pharmacol. Exp. Ther. 277:661‐70
后,动物的杂合性逐渐降低,因而纯度逐渐增高,使基因位点变为纯合子,使它们的表现型趋向一致性,如A与a基因频率相同,但基因型分配变化,经过一定代数的兄妹交配,则不再有杂合子,而获得遗传稳定的AA与aa近交品系,见表3-1。同胞兄妹或亲子交配连续20代以上就培育成近交系,为了保持该系的遗传特点,必须继续近交,并不得因突变或遗传漂移而丢失。 表3-1 近交引起的基因纯合情况 P(A)=0.5 p(a)=0.5 代数 各 种 基 因 的 比 例 纯合子(野鼠色)AA
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