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Eno1/Eno1蛋白Recombinant Mouse A

lpha-enolase (Eno1)重组蛋白2-phospho-D-glycerate hydro-lyase Enolase 1 Non-neural enolase蛋白
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  • ¥1836 - 14796
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  • CSB-EP007670MOb0
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Mouse Alpha-enolase (Eno1), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥14796.0
    规格:100μg产品价格:¥3468.0
    规格:20μg产品价格:¥1836.0

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    基因名:

    Eno1

    Uniprot No.:

    P17182

    别名:

    2-phospho-D-glycerate hydro-lyase Enolase 1 Non-neural enolase

    种属:

    Mus musculus (Mouse)

    蛋白长度:

    Partial

    来源:

    E.coli

    分子量:

    50.4 kDa

    表达区域:

    2-433aa

    氨基酸序列:

    SILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLA

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    In Stock Protein

    Biological_Activity:

    /

    Research Areas:

    Signal Transduction

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    "Cholesteryl ester loading of mouse peritoneal macrophages is associated with changes in the expression or modification of specific cellular proteins, including increase in an alpha-enolase isoform." Bottalico L.A., Kendrick N.C., Keller A., Li Y., Tabas I. Arterioscler. Thromb. 13:264-275(1993)

    Function:

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (By similarity). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (By similarity).

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    图标文献和实验
    该产品被引用文献
    "Cholesteryl ester loading of mouse peritoneal macrophages is associated with changes in the expression or modification of specific cellular proteins, including increase in an alpha-enolase isoform." Bottalico L.A., Kendrick N.C., Keller A., Li Y., Tabas I. Arterioscler. Thromb. 13:264-275(1993)
    相关实验
    • 【求助】关于gsk3-beta功能的疑问

      下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。

    • [资源] 所有的看家基因(housekeeping genes)列表+引物设计服务

      2 ganglioside activator protein (GM2A), mRNA 1250 NM_000967 Homo sapiens ribosomal protein L3 (RPL3), mRNA 7416 NM_001428 Homo sapiens enolase 1, (alpha) (ENO1), mRNA 3668 NM_000999 Homo sapiens ribosomal protein L38 (RPL38 ), mRNA 8302 NM_000997

    • 转载 层析复性蛋白

      层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源

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    文献支持
    Eno1/Eno1蛋白Recombinant Mouse Alpha-enolase (Eno1)重组蛋白2-phospho-D-glycerate hydro-lyase Enolase 1 Non-neural enolase蛋白
    ¥1836 - 14796