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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-7 protein (IL7) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10μg
英文名称:
Recombinant Human Interleukin-7 protein (IL7) (Active)品名简称:
Recombinant Human IL7 protein (Active)货号:
CSB-AP001751HU规格:
10μg价格:
2124LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. rHuIL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is > 1.0 × 10^8 Units/mg基因名:
IL7Uniprot No.:
P13232别名:
IL-7,种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
17.4 kDa表达区域:
26-177aa氨基酸序列:
DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.功能2:
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.文献:
/研究领域:
Immunology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验,以前曾以淋巴细胞活化因子( lymphocyte activating factor)。细胞促进蛋白质( mitogenic protein)以及 B细胞活化因子( B cell- activating factor)等七种名称称之。而 IL2属于淋巴细胞活素,以前曾以胸腺细胞刺激因子( thymocyte stimulating factor)、 T细胞生长因子( T cell growth factor)等六种名称称之。
【讨论】Innate immunity: 'Natural helper' cells identified
lineage (LIN) markers but express interleukin-7 receptor-α (IL-7Rα) and ST2 (a subunit of IL-33R), along with several other cell surface molecules. Further analysis showed that these cells are of lymphoid lineage but are distinct from B and T cells
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
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