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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Interleukin-2 protein (Il2) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
500μg/100μg/5μg
| 规格: | 500μg | 产品价格: | ¥14652.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6528.0 |
| 规格: | 5μg | 产品价格: | ¥852.0 |
纯度:
>95% as determined by SDS-PAGE.基因名:
Il2,Il-2Uniprot No.:
P04351别名:
IL-2, TCGF种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
17.2 kDa表达区域:
21-169aa氨基酸序列:
APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.内毒素:
Less than 1.0 EU/µg as determined by LAL method.SDS-PAGE:
/LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.Research Areas:
ImmunologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验TITLE Organization and structure of the mouse interleukin-2 gene JOURNAL Nucleic Acids Res. 12 (24), 9323-9331 (1984) PUBMED 6240025 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence
设计的引物TM在60度左右,最重要是扩增片段要在340-355bp,谢了先 FEATURES Location/Qualifiers source 1..939 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /chromosome="3" /map="3 19.2 cM" gene 1..939 /gene="Il2" /note="synonym: Il-2" /db_xref
and the cause of decreased refolding yields were included. Renaturation yield of recombinant protein have been improved by using some additives, such as molecular chaperone, small molecules. Key words inclusion body , protein , renaturation 外源基因在大肠杆菌中的
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