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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rhesus Macaque Interleukin-1 beta protein (IL1B) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10μg
英文名称:
Recombinant Rhesus Macaque Interleukin-1 beta protein (IL1B) (Active)品名简称:
Recombinant Rhesus macaque IL1B protein (Active)货号:
CSB-AP003081MOW规格:
10μg价格:
2124LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>98% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10.G4.1 cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.基因名:
IL1BUniprot No.:
P48090别名:
IL-1 beta,种属:
Macaca mulatta (Rhesus macaque)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
17.3 kDa表达区域:
117-269aa氨基酸序列:
APVRSLHCTLRDAQLKSLVMSGPYELKALHLQGQDLEQQVVFSMSFVQGEESNDKIPVALGLKAKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTRGGQDITDFTMQFVSS蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.功能2:
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.文献:
/研究领域:
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文献和实验的关系理不出头绪。GSK-3 beta 总蛋白减少,p-GSK-3 beta (ser 9)增加,是不是足以说明有活性的GSK-3 beta 减少更多,这样理解不知道对不对,希望给以指点。 galaxy22 楼主你好,请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的,active B catenin是减少的,那我有必要做ser9 和tyr216的磷酸化形式吗?我想研究wnt和akt。。。 棉花糖的温柔
attenuator 有一个问题一直没有查出相关资料或信息。 就是:β globin 蛋白在血液组织之外的细胞有没有表达,能不能RT检测出transcripts或western检测出protein。 为了这个去重复实验,条件不允许,但前人肯定有做过的,教科书里没有介绍,只介绍了时间特异性(胚胎到成体),没有介绍组织特异性——除了造血组织(或成红祖细胞)之外,在其他组织里,β globin是不是就沉默了,它那一簇基因是不是从它LCR
A Guide to Yeast Two-Hybrid Experiments(酵母双杂交实验指南)
*Correspondence: rfinley@wayne.edu Introduction The yeast two-hybrid system is a powerful genetic assay for protein-protein interactions (Chien et al., 1991; Fields and Song, 1989). The system has been used in thousands of studies
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