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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-1 alpha protein (IL1A) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10μg
英文名称:
Recombinant Human Interleukin-1 alpha protein (IL1A) (Active)品名简称:
Recombinant Human IL1A protein (Active)货号:
CSB-AP001661HU规格:
10μg价格:
2124LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>97% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 × 109 IU/mg.基因名:
IL1AUniprot No.:
P01583别名:
IL-1 alpha,种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
18.0 kDa表达区域:
113-271aa氨基酸序列:
SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.功能2:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.文献:
/研究领域:
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文献和实验癌、卵巢肿瘤、恶性畸胎瘤、胰腺癌、肠癌、肺癌等患者。 产甲胎蛋白的胃癌 (alpha-fetoprotein-producing gastric carcinoma, AFPGC) 是一种相对少见的胃癌 (GC),最早由 Bourreille 等人 [6] 于 1970 年提出,高 AFP 水平的胃癌被称为 AFPGC [7]。AFPGC 约占 GC 的 2.7~8%[8],也有报道占 GC 的 5.1%~15%[9]。AFPGC 是指经过病理或组织学检查确诊,伴有血清 AFP 升高,并可以排除肝炎
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
「铲屎官」注意了!剑桥大学揭示这些基因的改变或让猫猫狗狗成为
细胞 iBMDM 和犬的巨噬样细胞 DH82。结果表明小鼠巨噬细胞感染 2 小时后开始裂解,而犬的巨噬样细胞经过 12 小时仍存活,表明犬的细胞对于鼠伤寒沙门菌的耐受性更高。接着,研究者在小鼠模型上通过 CRISPR-Cas9 技术构建了与 caspase-1/-4 融合蛋白催化作用相当的重组蛋白。小鼠细胞在脂多糖(LPS)刺激下很快裂解,而犬的巨噬样细胞对于 LPS 的刺激则无反应。进一步的体外实验表明,caspase-1/-4 融合蛋白能激活 IL-1β 并且同时切割消皮素 D。图片来源:Cell
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