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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Fibroblast growth factor 21 protein (FGF21) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
5μg
英文名称:
Recombinant Human Fibroblast growth factor 21 protein (FGF21) (Active)品名简称:
Recombinant Human FGF21 protein (Active)货号:
CSB-AP002471HU规格:
5μg价格:
852LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>96% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin.基因名:
FGF21Uniprot No.:
Q9NSA1别名:
FGF-21,种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
19.4 kDa表达区域:
29-209aa氨基酸序列:
HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 µm filtered PBS, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. {ECO:0000269|PubMed:15902306, ECO:0000269|PubMed:17623664}.功能2:
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.文献:
/研究领域:
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文献和实验Metabolism 研究内容 FGF21 是一种由多种代谢应激在肝脏中诱导的激素,包括饥饿、蛋白质缺乏、单糖和乙醇等各种刺激。它作用于一个异聚细胞表面受体,由一个传统的 FGF 受体酪氨酸激酶(FGFR1c)与单通道跨膜蛋白 b-Klotho(KLB)复合物组成。 在人类中,乙醇是迄今为止所发现的、最有效的 FGF21 诱导剂。先前的研究表明,FGF21 能够抑制乙醇偏好,诱导个体自主饮水以防止脱水,并防止酒精诱导的肝损伤。 为了探究 FGF21 存在的重要性,本研究给野生型(WT)和 FGF21
明明是必须氨基酸,吃了却有害健康,喻德阳等 Cell 子刊揭示其中代谢机制
Metabolism 最后,作者分析了人类异亮氨酸摄入与 BMI 之间的关系。不出所料,异亮氨酸的摄入量与人体的 BMI 呈现出了显著的相关性。表明异亮氨酸在食物蛋白中的含量,可能是人类代谢健康的重要调节者。 研究意义: 图片来源:Cell Metabolism 该研究首次揭露了低蛋白饮食是如何帮助人体获得健康收益的,并直接锁定到了异亮氨酸与缬氨酸。同时,作者还进行了大量实验排除了传统氨基酸感应通路的影响,并通过组学分析,发现了 FGF21-UCP1 通路在异亮氨酸所调节的代谢过程中的关键作用,为他们的发现提供
健康的减肥药真的要来了?降体重、消炎症,还能改善代谢健康,或成为减肥者福音!
迁移、免疫调节和免疫效应反应等。 这些发现反映了 ARG2 过表达后的肝脏出现了能量消耗增加和抗炎表型。此外,这些肝脏代谢和炎症的改善与诱导产生的 LC3B-II 和 FGF21 蛋白在肝脏和血清中的积累有关,后两者为肝细胞禁食和应激反应的生物标志物。 图片来源:Cell Reports Medicine 药物介导的精氨酸分解代谢可诱导糖尿病小鼠产热和胰岛素耐受 上述的实验证明,肝细胞特异性 arcA 表达可显著改善遗传性糖尿病小鼠的胰岛素抵抗、糖耐受性和血脂异常。ADI-PEG
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