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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Desert hedgehog protein (DHH), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
5μg
英文名称:
Recombinant Human Desert hedgehog protein (DHH), partial (Active)品名简称:
Recombinant Human DHH protein, partial (Active)货号:
CSB-AP000411HU规格:
5μg价格:
852LC-MS/Western Blot:
/SDS-PAGE:
/纯度:
>96% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 μg/ml.基因名:
DHHUniprot No.:
O43323别名:
DHH,种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.Coli分子量:
19.9 kDa表达区域:
24-198aa氨基酸序列:
II+GPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGG蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered 10mM PB, pH 6.0, 300mM NaCl储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.功能2:
Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.文献:
/研究领域:
Stem Cells请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Purifying the Hedgehog Protein and its Variants
to human Indian and Desert hedgehog N-terminal signaling proteins, and therefore should be useful for hedgehog proteins from other species.
. Others rely on the transgenic expression of longer dsRNA molecules, leaving it to the cellular machinery to process these hairpins into short active dsRNA. In this chapter, we describe a transgenic method to deplete a chosen protein from a specific
1、做液闪仪测心肌细胞线粒体ANT转运酶活性检测,请问有没有非超声破膜的方法(没超声破碎仪)?2、若提得线粒体,如何看它有没有活性呢,用詹那绿B 就可以么?3、液闪仪大概需要多少样本量?丁香园网友dhh认为:提取新鲜心肌组织细胞内线粒体的方案:1、心肌组织切碎后在4 ℃介质(0.25 mol/L蔗糖、10 mmol/L Tris-HCl pH7.4,0-4℃)中制备心肌组织匀浆。匀浆经750g、离心10 min后留上清,以9000 g离心20 min 后留沉淀,重新悬浮后以9000 g再离心
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