Product overview Description Anti-LEFTY2 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Full length fusion protein Reactivity Human, Mouse Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol LEFTY2 Full name left-right determination factor 2 Synonyms EBAF; LEFTA; TGFB4; LEFTYA Swissprot O00292
Target Background This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. Alternate splicing of this gene results in multiple transcript variants.
Applications Immunohistochemistry Predicted cell location: Cytoplasm and Cell membrane Positive control: Human liver cancer Recommended dilution: 10-50 Predicted cell location: Cytoplasm and Cell membrane Positive control: Human prostate cancer Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223312(LEFTY2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ml223312(LEFTY2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:41 kDa Positive control:Human fetal muscle tissue Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Human fetal muscle tissue Primary antibody: ml223312(LEFTY2 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds