Product overview Description Anti-TBC1D22A rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human TBC1D22A Reactivity Human, Mouse Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol TBC1D22A Full name TBC1 domain family, member 22A Synonyms C22orf4; HSC79E021 Swissprot Q8WUA7
Target Background TBC1D22A (TBC1 domain family, member 22A), also known as C22orf4, is a 517 amino acid protein that contains one Rab-GAP TBC domain and is thought to function as a GTPase-activating protein for Rab family members. Multiple isoforms of TBC1D22A exist due to alternative splicing events. The gene encoding TBC1D22A maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm Positive control: Human prostate cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223293(TBC1D22A Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ml223293(TBC1D22A Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:59 kDa Positive control:A431 cell and human lung cancer tissue Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: A431 cell and human lung cancer tissue Primary antibody: ml223293(TBC1D22A Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute