Product overview Description Anti-TBP rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Full length fusion protein Reactivity Human Content 0.8 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol TBP Full name TATA box binding protein Synonyms HDL4; GTF2D; SCA17; TFIID; GTF2D1 Swissprot P20226
Target Background This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
Applications Immunohistochemistry Predicted cell location: Nucleus Positive control: Human esophagus cancer Recommended dilution: 25-100 Predicted cell location: Nucleus Positive control: Human gastric cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223289(TBP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml223289(TBP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:38 kDa Positive control:231 cell,Human testis tissue,Hela and Hepg2 cell Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: 231 cell,Human testis tissue,Hela and Hepg2 cell Primary antibody: ml223289(TBP Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds