Product overview Description Anti-MAP3K7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human MAP3K7 Reactivity Human, Mouse, Rat Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol MAP3K7 Full name mitogen-activated protein kinase kinase kinase 7 Synonyms TAK1; MEKK7; TGF1a Swissprot O43318
Target Background The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Applications Immunohistochemistry Predicted cell location: Cytoplasm and Cell membrane Positive control: Human esophagus cancer Recommended dilution: 10-50 Predicted cell location: Cytoplasm and Cell membrane Positive control: Human lung cancer Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223285(MAP3K7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml223285(MAP3K7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:67 kDa Positive control:HEPG2 cell lysate Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: HEPG2 cell lysate Primary antibody: ml223285(MAP3K7 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute