Product overview Description Anti-STX18 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human STX18 Reactivity Human, Mouse, Rat Content 0.4 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol STX18 Full name syntaxin 18 Synonyms Ufe1 Swissprot Q9P2W9
Target Background Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain carboxy-terminal hydrophobic domains that direct themselves to their respective intracellular compartments. Syntaxin 18 mainly localizes to the endoplasmic reticulum (ER), and functions in transport between the ER and Golgi. Syntaxin 18 also plays a role in ER-mediated phagocytosis, possibly by regulating the specific fusion of the ER and plasma or phagosomal membranes.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm Positive control: Human breast cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223273(STX18 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml223273(STX18 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:39 kDa Positive control:Raw264.7 and TM4 cells Recommended dilution: 200-1000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Raw264.7, TM4 cells Primary antibody: ml223273(STX18 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds