Product overview Description Anti-PVRL1 rabbit polyclonal antibody Applications ELISA, WB Immunogen Synthetic peptide of human PVRL1 Reactivity Human, Mouse, Rat Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol PVRL1 Full name Poliovirus receptor-related protein 1 Synonyms ED4, PRR, HIgR, HVEC, OFC7, PRR1, PVRR, CD111, PVRR1, SK-12, CLPED1, nectin-1 Swissprot Q15223
Target Background This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.
Applications Western blotting Predicted band size:57 kDa Positive control:Human cervical cancer, legs fibrous histiocytoma and fetal brain tissue Recommended dilution: 200-1000
Gel: 10%SDS-PAGE Lysate: 30 μg Lane 1-3: Human cervical cancer tissue, Human legs fibrous histiocytoma, Human fetal brain tissue Primary antibody: ml260288(PVRL1 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute