- 询价
- gelatins
- JLC222953
- 上海
- 2025年07月13日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-PTRH2 antibody
- 宿主：
Rabbit
- 浓度：
0.1 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-PTRH2 antibody
Cat. No. JLC222953
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-PTRH2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human PTRH2
Reactivity Human, Mouse
Content 0.1 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PTRH2
Full name peptidyl-tRNA hydrolase 2
Synonyms BIT1; PTH2; CFAP37; CGI-147
Swissprot Q9Y3E5
Target Background
PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human ovarian cancer
Recommended dilution: 10-50 Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml222953(PTRH2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml222953(PTRH2 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:19 kDa
Positive control:K562, hepg2, 231, Jurkat and Raji cells
Recommended dilution: 500-2000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane 1-5: K562 cells, hepg2 cells, 231 cells, Jurkat cells, Raji cells
Primary antibody: ml222953(PTRH2 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-5000
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