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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Chlamydia trachomatis Probable outer membrane protein PmpD protein (1244-1531AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Biotin
- 适应物种:
Chlamydia trachomatis
- 保质期:
6个月
- 抗原来源:
Chlamydia trachomatis
- 目录编号:
O84818
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
pmpD
- 抗体英文名:
pmpD Antibody, Biotin conjugated
- 抗体名:
pmpD antibody;CT_812 antibody;Probable outer membrane protein PmpD antibody;Polymorphic membrane protein D antibody
- 规格:
100μl/50μl
| 规格: | 100μl | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
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文献和实验Coupling of Monoclonal Antibodies with Biotin
The avidin-biotin bond is the strongest known biological interaction between a ligand and a protein ( K d = 1.3 � 10 −15 M at pH 5) ( 1 ). The affinity is so high that the avidin-biotin
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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