- 询价
- gelatins
- JLC222749
- 上海
- 2025年07月16日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-NDUFS4 antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-NDUFS4 antibody
Cat. No. JLC222749
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NDUFS4 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NDUFS4
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NDUFS4
Full name NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa
Synonyms AQDQ; CI-18
Swissprot O43181
Target Background
This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222749(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml222749(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:20 kDa
Positive control:Mouse kidney and heart tissue
Recommended dilution: 500-2000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Mouse kidney tissue, Mouse heart tissue
Primary antibody: ml222749(NDUFS4 Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-5000
Cat. No. JLC222749
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NDUFS4 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NDUFS4
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NDUFS4
Full name NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa
Synonyms AQDQ; CI-18
Swissprot O43181
Target Background
This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222749(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml222749(NDUFS4 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:20 kDa
Positive control:Mouse kidney and heart tissue
Recommended dilution: 500-2000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Mouse kidney tissue, Mouse heart tissue
Primary antibody: ml222749(NDUFS4 Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-5000
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Anti-NDUFS4 antibody
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