KLH conjugated synthetic peptide derived from human WIF1
规格:
0.2ml/200μg
Wnt信号抑制因子1抗体说明书CL-0144LoVo(人大肠癌细胞)5×106cells/瓶×2 GPT2 Others Rat 大鼠 GPT2 杆状病毒-昆虫细胞裂解液 (阳性对照) 人肾系膜细胞裂解物HRMCL SK-OV-3[SKOV-3]细胞,人卵巢癌细胞 人支气管上皮细胞,HBE细胞 脑膜细胞Many types of cells包装:5 × 105次方(1ml) 兔角膜前基质层成纤维细胞;RCFBF TEK Others Human 人 Tie2 / CD202b 人细胞裂解液 (阳性对照) 英文名称 Anti-WIF1 中文名称 Wnt信号抑制因子1抗体说明书 别 名 WIF-1; wif1; WIF1_HUMAN; Wnt Inhibitory Factor 1.
浓 度 1mg/1ml 规 格 0.2ml/200μg 抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse 产品类型 一抗 研究领域 肿瘤 免疫学 信号转导 转录调节因子 蛋白分子量 predicted molecular weight: 39kDa 性 状 Lyophilized or Liquid 免 疫 原 KLH conjugated synthetic peptide derived from human WIF1 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 产品介绍 The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the transmembrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation. Reduced levels of active GSK causes accumulation of b-catenin and subsequent regulation of developmentally significant Wnt target genes. Wnt antagonists such as dickkopf (Dkk), frizzled-related protein (sFRP) and Wnt inhibitory factor-1 (WIF-1), are necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes. WIF-1 is a 379-amino acid secreted protein that contains an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats and a 45-amino acid C-terminal hydrophilic domain. Subcellular Location : Secreted.