Product overview Description Anti-HSPA9 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human HSPA9 Reactivity Human, Mouse, Rat Content 0.6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol HSPA9 Full name heat shock 70kDa protein 9 (mortalin) Synonyms CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m Swissprot P38646
Target Background This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human brain Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222450(HSPA9 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml222450(HSPA9 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:74 kDa Positive control:HT-29, A549, 293T, MCF-7 and Hela cells Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-5: HT29 cells, A549 cells, 293T cells, MCF7 cells, Hela cells Primary antibody: ml222450(HSPA9 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds