Product overview Description Anti-DRG1 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human DRG1 Reactivity Human, Mouse Content 0.6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol DRG1 Full name developmentally regulated GTP binding protein 1 Synonyms NEDD3 Swissprot Q9Y295
Target Background DRG1 (developmentally regulated GTP binding protein 1), also known as NEDD3 (neural precursor cell expressed developmentally down-regulated protein 3), is a 367 amino acid protein that localizes to the cytoplasm and belongs to the GTP1/OBG family. Expressed at high levels in heart, kidney and skeletal muscle and at lower levels in brain, liver, placenta, lung, colon and spleen, DRG1 binds to TAL1 and TAL2 and is thought to play a role in cell proliferation and differentiation, as well as in apoptosis, suggesting a role in tumor formation and metastasis. DRG1 is subject to polyubiquitination and sumoylation, the former of which induces proteolytic degradation. The gene encoding DRG1 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human tonsil Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml222256(DRG1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222256(DRG1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:41 kDa Positive control:Human fetal brain tissue Recommended dilution: 500-2000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane: Human fetal brain tissue Primary antibody: ml222256(DRG1 Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds