- 询价
- gelatins
- JLC222111
- 上海
- 2025年07月12日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-WNT3A antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC222111
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-WNT3A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human WNT3A
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol WNT3A
Full name wingless-type MMTV integration site family, member 3A
Synonyms
Swissprot P56704
Target Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222111(WNT3A Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml222111(WNT3A Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:39 kDa
Positive control:A549 cells
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: A549 cells
Primary antibody: ml222111(WNT3A Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-2000
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