LS-F8635 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GLI / GLI1 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of GLI / GLI1 as low as 0.119 nanograms per millilter.
GLI Family Zinc Finger Protein 1 (GLI1) No significant cross-reactivity or interference between GLI Family Zinc Finger Protein 1 (GLI1) and analogs was observed.
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.313 - 20 ng/ml
Sensitivity
0.119 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components