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文献和实验Apoptosis Measurement by Annexin V Staining
assay. Recently, PARP cleavage (7 ) and annexin V (8 –11 ) have claimed more specificity. PARP cleavage occurs early in the response as a result of the activity of caspase-3 and correlates well with chromatin condensation (12 ). PARP cleavage
Annexin V/7-AAD Staining in Keratinocytes
Annexin V/7-amino-actinomycin staining is a convenient way to discriminate early apoptosis from late apoptosis and necrosis. Early apoptotic cells express phosphatidylserines (PS) on the outer leaflet of the plasma membrane. PS can be stained
. During apoptosis, PS is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. 实验步骤 Example
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