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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Mouse, Human, Rat
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
详见官方网站
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F6 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GHRL / Ghrelin Preproprotein in samples of Cell Culture Supernatants, Cell Lysates, Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of GHRL / Ghrelin Preproprotein as low as 0.1 nanograms per millilter.Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
GHRL / Ghrelin Preproprotein
Reactivity
Mouse, Human, Rat
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Nesfatin, Angiotensin II, NPY and APC.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.1 - 1000 ng/ml
Sensitivity
0.1 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About GHRL / Ghrelin Preproprotein
Q9UBU3 NM_016362 NP_057446.1
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Competition ELISA Platform Overview
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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